OPTICAL IMAGING FOR STEM CELL RESEARCH
Stem cell research requires a wide range of skills in order to study the entire process of cell selection, isolation, modification, inoculation, tracking and fate monitoring. This last step of observation of the stem cells after injection in the animal is challenging: it requires being able to monitor the cells:
At the organism level to follow their migrations and at a more precise level to estimate cell survival, proliferation and ideally differentiation. Yet determining the stem cell fate is the key point of all the work performed beforehand. The PhotonIMAGER™ offers you decisive advantages in this quest for versatility and precision.
High sensitivity to track cell migration pathways
The design of the PhotonIMAGER™, with its intensified CCD camera, makes it really sensitive from the first second of the acquisition makes it the perfect solution for cell tracking experiments.
Excellent linearity to correlate signal intensity with cell proliferation estimation
Users of the PhotonIMAGER™ can rely on its outstandingly large dynamic range: Stem cells or migrating cells luminescence expression can therefore be followed and compared, with the possibility to compare cell proliferation and cell fate with time, or between individuals when the cells are located in similar locations.
Versatility to offer fields of view from whole body scale to the organ level
Being able to detect your migrating cells by whole body imaging of your mouse, in vivo and non invasively, is great. Yet it becomes really exciting only when you can have a closer look at the cells, to localize or quantify them more precisely. The PhotonIMAGER™, with its moving stage, allows you to really focus on the fate of your cells.
Fluorescence imaging of neuronal precursors
The acquisition focused on the signal of the mouse brain; the signal was co-registered with a mouse brain photography for co-localization. The detected signal is consistent with the presence of large number of fluorescent neuron precursors in the subventricular zone and in the olfactory bulb.
Courtesy of Dr. Couillard Desprès and Pr. L. Aigner, University of Regensburg, Germany
|Neuron precursors migrate from the subventricular zone to the olfactory bulb in the mouse brain (left mouse). The acquisition was performed without craniotomy, the cells could be detected through the skull of the animal, after shaving.The right mouse was used as control.
Courtesy of Dr. Couillard Desprès and Pr. L. Aigner, University of Regensburg, Germany.
Non-invasive imaging to follow migration longitudinally
If cell migration could be studied in vitro in Petri dishes, it would be so much easier. Yet animal models are necessary to have a physiological environment, with all the interactions and factors relevant to a living organism. Then if one needs to disturb this organism for the purpose of imaging, isn’t it going backwards?
The PhotonIMAGER™ was designed to help you provide your animals the best physiological conditions, to minimize disturbance. The system has a heating table, the temperature of which can be adapted; and anesthesia is performed with gas or injection, for your convenience. And with the In Actio imaging mode, you can even get rid of anesthesia, if this is compatible with your protocols!