FimH-based display of functional eukaryotic proteins on bacteria surfaces

In this report the scientific team from Center for Molecular Medicine Cologne (CMMC) present a novel FimH-based, IPTG-inducible expression system to display fully functional eukaryotic proteins on bacterial outer membranes. EGFR ligand (HRV3C) fusion proteins could be easily harvested from cell culture supernatants after proteolitic cleavage. In addition, tumor infiltration of GLuc-FimH bacteria could be tracked in vivo using the PhotonIMAGERTM RT.

Markus Chmielewski, Johannes Kuehle, Danuta Chrobok, Nicole Riet, Michael Hallek & HinrichAbken.

DOI: 10.1038/s41598-019-44883-z

PUBLICATION ALERT : Nature Cell Biology - EPFL - Lausanne.

Chemotherapy elicits pro-metastatic extracellular vesicles in breast cancer models

In this high impact scientific communication the authors demonstrate how, contrary to expectations, the two chemotherapy agents, Paclitaxel (PTX) and Doxorubicin (DOX) can promote tumor metastasis by inducing release of extracellular vesicles (EVs) from primary tumors. They used the PhotonIMAGERTMOPTIMA to demonstrate increased tumour growth in WT mice pre-conditioned with EVs prepared from 4T1 tumor bearing mice, which had been treated with PTX (PTX-EVs) compared to control CREMO-EVs. In contrast, Ccr2-KO mice did not show increase tumor growth when treated with PTX-EVs, thus suggesting a role for monocyte chemotaxis in PTX-EV mediated tumour enhancement. The researchers also observed enrichment of the Ca2+-dependant membrane-binding protein Annexin A6 (ANXA6) in PTX-EVs and DOX-EVs. ANXA6 is known to regulate membrane trafficking and cell signalling. The team then showed that the pro-metastatic capacity of chemotherapy-elicited EVs was dependent on Ly6C+CCR2+ monocytes and Annexin VI expression.

Ioanna Keklikoglou, Chiara Cianciaruso, Esra Güç2, Mario Leonardo Squadrito, Laura M. Spring3, Simon Tazzyman, Lore Lambein, Amanda Poissonnier, Gino B. Ferraro, Caroline Baer, Antonino Cassará, Alan Guichard, M. Luisa Iruela-Arispe, Claire E. Lewis,Lisa M. Coussens, Aditya Bardia, Rakesh K. Jain, Jeffrey W. Pollard and Michele De Palma

DOI: 10.1038/s41556-018-0256-3

Sensitivity of pre-targeted immunoPET using 68Ga-peptide to detect colonic carcinoma liver metastases in a murine xenograft model: Comparison with 18FDG PET-CT

In this report the scientific team from CRCINA, INSERM Nantes demonstrated the improved performance of pretargeted immunoPET imaging with 68Ga-probes compared to gold standard 18FDG-PET in an orthotopic murine xenograft model of human colonic liver metastases. They used the PhotonIMAGERTM RT system to validate tumor implantation post intra-portal injection of luciferase transfected LS174T cells in nude mice and quantify Hepatic tumor burden prior to PET imaging.

Fanny Foubert, Sébastien Gouard, Catherine Saï-Maurel, Michel Chérel, Alain Faivre-Chauvet, David M. Goldenberg, Jacques Barbet, Clément Bailly, Caroline Bodet-Milin, Thomas Carlier, Françoise Kraeber-Bodéré, Yann Touchefeu and Eric Frampas.

DOI: 10.18632/oncotarget.25514


Oral vitamin-A-coupled valsartan nanomedicine: High hepatic stellate cell receptors accessibility and prolonged enterohepatic residence

In this scientific report, a scientific team from Alexandria, Egypt, studied the therapeutic potentials of orally administered Vitamin A (VA) coupled Valsartan-loaded liposomes (VLCs) in a rat model of CCL4-induced liver fibrosis. They used the PhotonIMAGERTM OPTIMA to quantify relocation of Nile Red labelled VA coupled VLCs from the intestine to the liver upon onset of fibrosis for several days by Ex vivo fluorescence imaging. Confocal microscopy examination of immunofluorescently-stained HSCs in liver sections confirmed nuclear uptake of VLCs in hepatic stellate cells, in accordance with Valsartan’s mode of action. The authors concluded that VA coupled nanocarriers represented a promising targeted delivery method for oral administration of Valsartan in antifibrotic therapy.

Nesrine S. El-Mezayen, Wessam F. El-Hadidy, Wessam M. El-Refaie, Thanaa I. Shalaby, Mahmoud M. Khattab, Aiman S. El-Khatib

DOI: 10.1016/j.jconrel.2018.05.021


The histone demethylase Phf2 acts as a molecular checkpoint to prevent NAFLD progression during obesity.

In this report the french scientific team from the INSERM, U1016/CNRS, UMR8104 studied the involvement of aberrant histone methylation in Non Alcoholic Fatty Liver Disease (NAFLD). They identified the histone demethylase Plant Homeodomain Finger 2 (Phf2) as a new co-activator of the transcription factor Carbohydrate Responsive Element Binding Protein (ChREBP) and established its role as a downstream epigenetic checkpoint, protecting the liver from the pathogenesis progression through facilitating H3K9me2 demethylation at specific gene promoters.

They used the PhotonIMAGERTM system to compare activation of ChREBP-regulated LPK-Luc and SCD1-Luc reporter genes in the liver of control and Phf2 overexpressing mice and follow inflammation by monitoring signal from the Peroxy Caged Luciferin-2 (PCL-2) biosensor in wild type and Nrf2-KO mice overexpressing Phf2.

The Authors concluded that Phf2 facilitates H3K9me2 demethylation at ChREBP regulated-gene promoters and protects liver from the accumulation of pathogenic lipids and ROS during NAFLD.

Julien Bricambert, Marie-Clotilde Alves-Guerra, Pauline Esteves, Carina Prip-Buus, Justine Bertrand-Miche, Hervé Guillou , Christopher J. Chang, Mark N. Vander Wal, François Canonne-Hergaux, Philippe Mathurin, Violeta Raverdy, François Pattou, Jean Girard, Catherine Postic & Renaud Dentin.

DOI: 10.1038/s41467-018-04361-y

PUBLICATION ALERT : SCIENCE ADVANCES - Karolinska institute, Sweden.

Mannan-induced Nos2 in macrophages enhances IL-17–driven psoriatic arthritis by innate lymphocytes.

In this report, the authors studied the pathogenic role of Nitric Oxide (NO) in a mouse model for mannan induced psoriasis and psoriatic arthritis (MIP).

In particular, they investigated the effect of inhibition of the nitric oxide synthase (NOS2) and/or the nicotinamide adenine dinucleotide phosphate oxidase 2 (NOX2) on mannan-induced NO release.

Mannan-induced NO production was measured indirectly using Luminol-based L-012, which react with peroxynitrite, a down-product of NO. L-012 chemiluminescence was quantified with the Photon IMAGERTM Optima.

Inhibition of NOS2 with L-NAME or its less efficient analogue D-NAME, significantly reduced L-012 signal. Furthermore, impairment of NOX2-mediated superoxide anion production in Ncf1*/* mutant mice also significantly reduced the L-012 chemiluminescent signal.

The authors concluded that Mannan NO production can be induced by mannan in a NOS2 dependent manner and that mannan-induced NO-derived peroxynitrite is dependent on NOX2-derived superoxide.

Jianghong Zhong, Tatjana Scholz, Anthony C. Y. Yau, Simon Guerard, Ulrike Hüffmeier,Harald Burkhardt, Rikard Holmdahl.


The ceramide transporter CERT is involved in muscle insulin signaling defects under lipotoxic conditions.

In this scientific report, the authors investigated the importance of CERT in lipid induced insulin resitance .

Amongst other experiments, the researchers used digital autoradiography to study the effect of C2C12 myotubes treatment with the CERT inhibitor HPA12 on [3H]-Sphingosine conversion to [3H]-ceramide, [3H]-Sphingomyosine and [3H]-GlcCer.
C2C12 lipid extracts resolved by High performance Liquid chromatography were quantified by direct analysis of the HTPLC plates on the BetaIMAGER Tracer.

The results showed that most of the [3H]-sphingosine down-products were associated with ceramide and that CERT inhibition with HPA12 or palmitate treatment substantially reduced sphingomyelin (SM) production and no effect on Ceramide or GlcCer biosynthesis.

Cécile L. Bandet, Rana Mahfouz, Julien Véret, Athanassia Sotiropoulos, Maxime Poirier, Paola Giussani, Mélanie Campana, Erwann Philippe, Agnieszka Blachnio-Zabielska, Raphaëlle Ballaire, Xavier Le Liepvre, Olivier Bourron, Dušan Berkeš, Jan Górski, Pascal Ferré, Hervé Le Stunff, Fabienne Foufelle and Eric Hajduch*

DOI: 10.2337/db17-0901


Anesthesia Inhibits the Activity of the "Glymphatic System”

In this report, the researchers from Caen University Hospital (CHU Caen) took advantage of the PhotonIMAGER RT’s intensified CCD, which can : " dramatically increase the signal to noise ratio of NIRF signals" thanks to its fluorescence photocounting mode. NIRF acquisition times could be reduced to 22 ms, allowing real-time in vivo NIRF imaging of freely moving mice (45 frames per second).

Using this functionality to investigate CSF circulation amid the glymphatic system hypothesis, the authors could observe a substancial increase in Indocyaning Green diffusion to the forebrain of non-anesthetized mice whilst the marker remained located in the cisterna magna of anesthetised animals. This findings, confirmed by MRI and ex vivo fluorometry analysis, strongly suggest that parenchymal CSF circulation is mainly active during wakefulness and significantly impaired during general anesthesia.

Clement Gakuba, Thomas Gaberel, Suzanne Goursaud, Jennifer Bourges, Camille Di Palma, Aurélien Quenault, Sara Martinez de Lizarrondo, Denis Vivien, Maxime Gauberti.


PUBLICATION ALERT : Nuclear Medicine Dept, University Hospital, Nantes - OncoTarget 2018

Comparison of Immuno-PET of CD138 and PET imaging with 64CuCl2 and 18F-FDG in a preclinical syngeneic model of multiple myeloma

In this report, the research team from University Hospital Nantes used the PhotonIMAGER RT to validate 64Cu-labeled anti-CD138 murine antibody PET localization Data in a multiple myeloma syngeneic mouse model.

Clément Bailly, Sébastien Gouard, Marie Lacombe, Patricia Remaud-Le Saëc, Benjamin Chalopin, Mickaël Bourgeois, Nicolas Chouin, Raphaël Tripier, Zakaria Halime, Ferid Haddad, Alain Faivre-Chauvet, Françoise Kraeber-Bodéré, Michel Chérel, and Caroline Bodet-Milin.


PUBLICATION ALERT : Inserm U1026, Bordeaux - Nature Scientific Reports - 2017

In situ printing of mesenchymal stromal cells, by laser-assisted bioprinting, for in vivo bone regeneration applications

In this report, researchers from Bordeaux university used the PhotonIMAGERTM RT to assess proliferation of multipotent bone marrow stromal precursor cells implanted in situ by laser assisted bioprinting (LAB) in a calvaria defect mouse model.

Virginie Keriquel, Hugo Oliveira, Murielle Rémy, Sophia Ziane, Samantha Delmond, Benoit Rousseau, Sylvie Rey, Sylvain Catros, Joelle Amédée, Fabien Guillemot & Jean-Christophe Fricain.

PUBLICATION ALERT : ETH Zurich - Psychopharmacology - 2017

The role of beta-arrestin2 in shaping fMRI BOLD responses to dopaminergic stimulation

In this report the authors used the digital autoradigraphy BetaIMAGER TRacer system to validate binding of tritiated ligands for for D1R, D2/3R, dopamine transporter (DAT) and vesdicular monoamine transporter-2 (VMAT2) in Wild-Type and beta-arrestin2 K/O mice before functional analysis by fMRI.

Kristoffer Sahlholm, Giovanna D. Ielacqua, Jinbin Xu, Lynne A. Jones, Felix Schlege, Robert H. Mach, Markus Rudin and Aileen Schroeter

DOI: 10.1007/s00213-017-4609-6

PUBLICATION ALERT : INSERM - Nature communications - 2017

Loss of HIF-1α in natural killer cells inhibits tumour growth by stimulating non-productive angiogenesis.

In this report the authors used the PhotonIMAGER RT system to quantifiy tumor hypoxia and angiogenesis non-invasevely after i.v. injection of the fluorescent markers AngioStamp®700 and HypoxiSense®680 in Wild Type and Hypoxia-inducible-factor-1-aplha deficient mice (HIF-1α K/O) bearing subcutaneous MC38 tumor isografts.

Ewelina Krzywinska, Chahrazade Kantari-Mimoun, Yann Kerdiles, Michal Sobecki, Takayuki Isagawa, Dagmar Gotthardt, Magali Castells, Johannes Haubold, Corinne Millien, Thomas Viel, Bertrand Tavitian, Norihiko Takeda, Joachim Fandrey, Eric Vivier, Veronika Sexl & Christian Stockmann.

PUBLICATION ALERT : VUB / UZB - Mol Imaging Biol 2017

Improved Debulking of Peritoneal Tumor Implants by Near-Infrared Fluorescent Nanobody Image Guidance in an Experimental Mouse Model.

In this report the scientific teams from UVB and UZ Brussels used the PhotonIMAGER  Optima to validate surgical efficiency of a debulking technique by fluorescence guided surgery using a NIR-labeled nanobody against HER2-positive tumors.



Pieterjan Debie, Marian Vanhoeij, Natalie Poortmans, Janik Puttemans, Kris Gillis, Nick Devoogdt, Tony Lahoutte, Sophie Hernot



Potential of Luminescence Based Molecular Animal Imaging in Research Areas Pertaining to Cancer Biology and Therapy

In this report, the researchers present the PhotonIMAGER RT system available in their laboratory for various cancer research activities including measurement of tumor growth kinetics, angiogenesis, therapeutic efficacy evaluation and metastasis studies. Moreover, the imaging system is also being used for radio-luminescence imaging based on Cherenkov radiation of radio-pharmaceuticals.

Hansa D. Yadav, Neena G. Shetake, Murali MS Balla, Amit Kumar and B.N. Pandey

ResearchGate link


PUBLICATION ALERT: Pharos University - Journal of Controlled Release

Hepatic stellate cell-targeted imatinib nanomedicine versus conventional imatinib: A novel strategy with potent efficacy in experimental liver fibrosis

In this study, novel VA-coupled imatinib-loaded liposomes (ILC) were prepared and optimized regarding VA-coupling efficiency, imatinib entrapment efficiency, and particle size. Preferential accumulation of the selected formula in liver was proved by tracing intraperitoneally (i.p.)-injected VA-coupled liposomes loaded with Nile Red (LCNR) to rats with CCl4-induced liver fibrosis.

Nesrine S. El-mezayen, Wessam F. El-Hadidy, Wessam M. El-Refaie, Th.I. Shalaby, Mahmoud M. Khattab, Aiman S. El-Khatib



PUBLICATION ALERT: Cardiff University - Molecular Biosciences Research Division . BIOTECHNOLOGY AND BIOENGINEERING.

ΔFlucs: Brighter Photinus pyralis firefly luciferases identified by surveying consecutive single amino acid deletion mutations in a thermostable variant

In this article, the authors used the PhotonIMAGER® OPTIMA system to analyse the emission spectra of consecutive single amino acid x11 Photinus firefly luciferase (Fluc) deletion mutants. Targeting of the ‘omega-loop’ motif (T352-G360) significantly enhanced activity, altered kinetics, altered emission colours and substrate specificity for luciferin substrates.

Lisa M. Halliwell, Amit P. Jathoul, Jack P. Bate,Harley L. Worthy, James C. Anderson, Dafydd D. Jones, James A. H. Murray

DOI: 10.1002/bit.26451


Publication Alert : CMIRO, Brussels /CMMI, Gosselies, Belgium - STEM CELLS AND DEVELOPMENT

Long-term In Vivo Monitoring of Adult-Derived Human Liver Stem/Progenitor Cells by Bioluminescence Imaging, Positron Emission Tomography, and Contrast-Enhanced Computed Tomography

In this article, a triple fusion reporter system (renilla luciferase/RFP/truncated HSV-1 thymidine kinase) was used monitor biodistribution of Adult-derived human liver stem/progenitor cells (ADHLSCs) injected in severe combined immunodeficiency/beige mice by BLI FLI and Positron emission tomography.

Mei-Ju Hsu, Julie Prigent, Pierre-Edouard Dollet, Joachim Ravau, Lionel Larbanoix, Gaetan Van Simaeys, Anne Bol, Vincent Gregoire, Serge Goldman, Gisele Deblandre, Mustapha Najimi, Etienne M. Sokal, and Catherine A. Lombard.

DOI: 10.1089/scd.2016.0338


Publication Alert : CEA - Cyceron - Caen - PLOSOne

[18F]Fludarabine-PET in a murine model of multiple myeloma

RPMI8226-GFP-Luc MM cells expressing the green fluorescent protein (GFP) as well as the luciferase reporter (Luc) were derived from the parental RPMI8226 cells and njected s.c. into the flank of nude mice. Myeloma tumour growth was followed using bioluminescence-based imaging (BLI) and characterised by immunohistochemistry (IHC). The tumour specificity of [18F]fludarabine was evaluated and compared to [18F]FDG. The team’s results suggest that [18F]fludarabine-PET might represent an alternative and perhaps more specific modality for Multiple Myeloma imaging when compared to [18F]FDG. Nevertheless, more investigations are required to extend this conclusion to humans.

Narinee Hovhannisyan, Martine Dhilly, Martin Fidalgo, Fabien Fillesoye, Stephane Guillouet, Brigitte Sola, Louisa Barre

DOI: 10.1371/journal.pone.0177125


Publication Alert : Keele University - Acta Biomaterialia

Defining a turnover index for the correlation of biomaterial degradation and cell basedextracellular matrix synthesis using fluorescent tagging techniques

Non-destructive protocols which can define a biomaterial’s degradation and its associated ability to support proliferation and/or promote extracellular matrix deposition will be an essential in vitro tool. In this study we investigate fluorescently tagged biomaterials, with varying rates of degradation and their ability to support cell proliferation and osteogenic differentiation. Changes in fluorescence of the biomaterials and the release of fluorescent soluble by-products were confirmed as accurate methods to quantify degradation. It was demonstrated that increasing rates of the selected biomaterials’ degradation led to a decrease in cell proliferation and concurrently an increase in osteogenic matrix production. A novel turnover index (TI), which directly describes the effect of degradation of a biomaterial on cell behavior, was calculated. Lower TIs for proliferation and high TIs for osteogenic marker production were observed on faster degrading biomaterials, indicating that these biomaterials supported an upregulation of osteogenic markers. This TI was further validated using an ex vivo chick femur model, where the faster degrading biomaterial, fibrin, led to an increased TI for mineralization within an epiphyseal defect. This in vitro tool, TI, for monitoring the effect ofbiomaterial degradation on extracellular matrix production may well act as predictor of the selected biomaterials’ performance during in vivo studies.

Katie Bardsley, Ian Wimpenny, Roni Wechsel, Yonatan Shachaf, Ying Yang, Alicia J.El Haj


New poster of Sanofi Pasteur for AFSTAL congress in Nantes

The PhotonIMAGER system to follow bacterial infections in mice in a non-invasive manner

This Sanofi Pasteur (Marcy-l’Étoile, France) study describes two new mouse models to follow the kinetics of infection with Pseudomonas aeruginosa and Staphylococcus aureus in longitudinal studies. Thanks to the Photon IMAGERTM system daily evaluation of the bacterial load was possible in vivo. Using a luciferase reporter gene expressed in both bacterial strains, direct correlation between CFUs and light emission could was assessed non-invasively and without any need for euthanasia or organ excision.

Focusing on the 3Rs principles, Sanofi Pasteur will be aiming to apply these models to current and future infectiology studies.



Dowload the poster

Implementation of murine infectious models using in vivo optical imaging

Sanofi Pasteur, Protection et Sciences de l’Animal, Marcy-l’Étoile, France






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