The ceramide transporter CERT is involved in muscle insulin signaling defects under lipotoxic conditions.

In this scientific report, the authors investigated the importance of CERT in lipid induced insulin resitance .

Amongst other experiments, the researchers used digital autoradiography to study the effect of C2C12 myotubes treatment with the CERT inhibitor HPA12 on [3H]-Sphingosine conversion to [3H]-ceramide, [3H]-Sphingomyosine and [3H]-GlcCer.
C2C12 lipid extracts resolved by High performance Liquid chromatography were quantified by direct analysis of the HTPLC plates on the BetaIMAGER Tracer.

The results showed that most of the [3H]-sphingosine down-products were associated with ceramide and that CERT inhibition with HPA12 or palmitate treatment substantially reduced sphingomyelin (SM) production and no effect on Ceramide or GlcCer biosynthesis.

Cécile L. Bandet, Rana Mahfouz, Julien Véret, Athanassia Sotiropoulos, Maxime Poirier, Paola Giussani, Mélanie Campana, Erwann Philippe, Agnieszka Blachnio-Zabielska, Raphaëlle Ballaire, Xavier Le Liepvre, Olivier Bourron, Dušan Berkeš, Jan Górski, Pascal Ferré, Hervé Le Stunff, Fabienne Foufelle and Eric Hajduch*

DOI: 10.2337/db17-0901


Anesthesia Inhibits the Activity of the "Glymphatic System”

In this report, the researchers from Caen University Hospital (CHU Caen) took advantage of the PhotonIMAGER RT’s intensified CCD, which can : " dramatically increase the signal to noise ratio of NIRF signals" thanks to its fluorescence photocounting mode. NIRF acquisition times could be reduced to 22 ms, allowing real-time in vivo NIRF imaging of freely moving mice (45 frames per second).

Using this functionality to investigate CSF circulation amid the glymphatic system hypothesis, the authors could observe a substancial increase in Indocyaning Green diffusion to the forebrain of non-anesthetized mice whilst the marker remained located in the cisterna magna of anesthetised animals. This findings, confirmed by MRI and ex vivo fluorometry analysis, strongly suggest that parenchymal CSF circulation is mainly active during wakefulness and significantly impaired during general anesthesia.

Clement Gakuba, Thomas Gaberel, Suzanne Goursaud, Jennifer Bourges, Camille Di Palma, Aurélien Quenault, Sara Martinez de Lizarrondo, Denis Vivien, Maxime Gauberti.

Real-Time Video 1.

Real Time movie 2.


PUBLICATION ALERT : Nuclear Medicine Dept, University Hospital, Nantes - OncoTarget 2018

Comparison of Immuno-PET of CD138 and PET imaging with 64CuCl2 and 18F-FDG in a preclinical syngeneic model of multiple myeloma

In this report, the research team from University Hospital Nantes used the PhotonIMAGER RT to validate 64Cu-labeled anti-CD138 murine antibody PET localization Data in a multiple myeloma syngeneic mouse model.

Clément Bailly, Sébastien Gouard, Marie Lacombe, Patricia Remaud-Le Saëc, Benjamin Chalopin, Mickaël Bourgeois, Nicolas Chouin, Raphaël Tripier, Zakaria Halime, Ferid Haddad, Alain Faivre-Chauvet, Françoise Kraeber-Bodéré, Michel Chérel, and Caroline Bodet-Milin.


PUBLICATION ALERT : Inserm U1026, Bordeaux - Nature Scientific Reports - 2017

In situ printing of mesenchymal stromal cells, by laser-assisted bioprinting, for in vivo bone regeneration applications

In this report, researchers from Bordeaux university used the PhotonIMAGERTM RT to assess proliferation of multipotent bone marrow stromal precursor cells implanted in situ by laser assisted bioprinting (LAB) in a calvaria defect mouse model.

Virginie Keriquel, Hugo Oliveira, Murielle Rémy, Sophia Ziane, Samantha Delmond, Benoit Rousseau, Sylvie Rey, Sylvain Catros, Joelle Amédée, Fabien Guillemot & Jean-Christophe Fricain.

PUBLICATION ALERT : ETH Zurich - Psychopharmacology - 2017

The role of beta-arrestin2 in shaping fMRI BOLD responses to dopaminergic stimulation

In this report the authors used the digital autoradigraphy BetaIMAGER TRacer system to validate binding of tritiated ligands for for D1R, D2/3R, dopamine transporter (DAT) and vesdicular monoamine transporter-2 (VMAT2) in Wild-Type and beta-arrestin2 K/O mice before functional analysis by fMRI.

Kristoffer Sahlholm, Giovanna D. Ielacqua, Jinbin Xu, Lynne A. Jones, Felix Schlege, Robert H. Mach, Markus Rudin and Aileen Schroeter

DOI: 10.1007/s00213-017-4609-6

PUBLICATION ALERT : INSERM - Nature communications - 2017

PUBLICATION ALERT : VUB / UZB - Mol Imaging Biol 2017

Improved Debulking of Peritoneal Tumor Implants by Near-Infrared Fluorescent Nanobody Image Guidance in an Experimental Mouse Model.

In this report the scientific teams from UVB and UZ Brussels used the PhotonIMAGER Optima to validate surgical efficiency of a debulking technique by fluorescence guided surgery using a NIR-labeled nanobody against HER2-positive tumors.



Pieterjan Debie, Marian Vanhoeij, Natalie Poortmans, Janik Puttemans, Kris Gillis, Nick Devoogdt, Tony Lahoutte, Sophie Hernot



Potential of Luminescence Based Molecular Animal Imaging in Research Areas Pertaining to Cancer Biology and Therapy

In this report, the researchers present the PhotonIMAGER RT system available in their laboratory for various cancer research activities including measurement of tumor growth kinetics, angiogenesis, therapeutic efficacy evaluation and metastasis studies. Moreover, the imaging system is also being used for radio-luminescence imaging based on Cherenkov radiation of radio-pharmaceuticals.

Hansa D. Yadav, Neena G. Shetake, Murali MS Balla, Amit Kumar and B.N. Pandey

ResearchGate link


PUBLICATION ALERT: Pharos University - Journal of Controlled Release

Hepatic stellate cell-targeted imatinib nanomedicine versus conventional imatinib: A novel strategy with potent efficacy in experimental liver fibrosis

In this study, novel VA-coupled imatinib-loaded liposomes (ILC) were prepared and optimized regarding VA-coupling efficiency, imatinib entrapment efficiency, and particle size. Preferential accumulation of the selected formula in liver was proved by tracing intraperitoneally (i.p.)-injected VA-coupled liposomes loaded with Nile Red (LCNR) to rats with CCl4-induced liver fibrosis.

Nesrine S. El-mezayen, Wessam F. El-Hadidy, Wessam M. El-Refaie, Th.I. Shalaby, Mahmoud M. Khattab, Aiman S. El-Khatib



PUBLICATION ALERT: Cardiff University - Molecular Biosciences Research Division . BIOTECHNOLOGY AND BIOENGINEERING.

ΔFlucs: Brighter Photinus pyralis firefly luciferases identified by surveying consecutive single amino acid deletion mutations in a thermostable variant

In this article, the authors used the PhotonIMAGER® OPTIMA system to analyse the emission spectra of consecutive single amino acid x11 Photinus firefly luciferase (Fluc) deletion mutants. Targeting of the ‘omega-loop’ motif (T352-G360) significantly enhanced activity, altered kinetics, altered emission colours and substrate specificity for luciferin substrates.

Lisa M. Halliwell, Amit P. Jathoul, Jack P. Bate,Harley L. Worthy, James C. Anderson, Dafydd D. Jones, James A. H. Murray

DOI: 10.1002/bit.26451


Publication Alert : CMIRO, Brussels /CMMI, Gosselies, Belgium - STEM CELLS AND DEVELOPMENT

Long-term In Vivo Monitoring of Adult-Derived Human Liver Stem/Progenitor Cells by Bioluminescence Imaging, Positron Emission Tomography, and Contrast-Enhanced Computed Tomography

In this article, a triple fusion reporter system (renilla luciferase/RFP/truncated HSV-1 thymidine kinase) was used monitor biodistribution of Adult-derived human liver stem/progenitor cells (ADHLSCs) injected in severe combined immunodeficiency/beige mice by BLI FLI and Positron emission tomography.

Mei-Ju Hsu, Julie Prigent, Pierre-Edouard Dollet, Joachim Ravau, Lionel Larbanoix, Gaetan Van Simaeys, Anne Bol, Vincent Gregoire, Serge Goldman, Gisele Deblandre, Mustapha Najimi, Etienne M. Sokal, and Catherine A. Lombard.

DOI: 10.1089/scd.2016.0338


Publication Alert : CEA - Cyceron - Caen - PLOSOne

[18F]Fludarabine-PET in a murine model of multiple myeloma

RPMI8226-GFP-Luc MM cells expressing the green fluorescent protein (GFP) as well as the luciferase reporter (Luc) were derived from the parental RPMI8226 cells and njected s.c. into the flank of nude mice. Myeloma tumour growth was followed using bioluminescence-based imaging (BLI) and characterised by immunohistochemistry (IHC). The tumour specificity of [18F]fludarabine was evaluated and compared to [18F]FDG. The team’s results suggest that [18F]fludarabine-PET might represent an alternative and perhaps more specific modality for Multiple Myeloma imaging when compared to [18F]FDG. Nevertheless, more investigations are required to extend this conclusion to humans.

Narinee Hovhannisyan, Martine Dhilly, Martin Fidalgo, Fabien Fillesoye, Stephane Guillouet, Brigitte Sola, Louisa Barre

DOI: 10.1371/journal.pone.0177125


Publication Alert : Keele University - Acta Biomaterialia

Defining a turnover index for the correlation of biomaterial degradation and cell basedextracellular matrix synthesis using fluorescent tagging techniques

Non-destructive protocols which can define a biomaterial’s degradation and its associated ability to support proliferation and/or promote extracellular matrix deposition will be an essential in vitro tool. In this study we investigate fluorescently tagged biomaterials, with varying rates of degradation and their ability to support cell proliferation and osteogenic differentiation. Changes in fluorescence of the biomaterials and the release of fluorescent soluble by-products were confirmed as accurate methods to quantify degradation. It was demonstrated that increasing rates of the selected biomaterials’ degradation led to a decrease in cell proliferation and concurrently an increase in osteogenic matrix production. A novel turnover index (TI), which directly describes the effect of degradation of a biomaterial on cell behavior, was calculated. Lower TIs for proliferation and high TIs for osteogenic marker production were observed on faster degrading biomaterials, indicating that these biomaterials supported an upregulation of osteogenic markers. This TI was further validated using an ex vivo chick femur model, where the faster degrading biomaterial, fibrin, led to an increased TI for mineralization within an epiphyseal defect. This in vitro tool, TI, for monitoring the effect ofbiomaterial degradation on extracellular matrix production may well act as predictor of the selected biomaterials’ performance during in vivo studies.

Katie Bardsley, Ian Wimpenny, Roni Wechsel, Yonatan Shachaf, Ying Yang, Alicia J.El Haj

New poster of Sanofi Pasteur for AFSTAL congress in Nantes

The PhotonIMAGER system to follow bacterial infections in mice in a non-invasive manner

This Sanofi Pasteur (Marcy-l’Étoile, France) study describes two new mouse models to follow the kinetics of infection with Pseudomonas aeruginosa and Staphylococcus aureus in longitudinal studies. Thanks to the Photon IMAGERTM system daily evaluation of the bacterial load was possible in vivo. Using a luciferase reporter gene expressed in both bacterial strains, direct correlation between CFUs and light emission could be assessed non-invasively and without any need for euthanasia or organ excision.

Focusing on the 3Rs principles, Sanofi Pasteur will be aiming to apply these models to current and future infectiology studies.



Dowload the poster

Implementation of murine infectious models using in vivo optical imaging

Sanofi Pasteur, Protection et Sciences de l’Animal, Marcy-l’Étoile, France






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