In this report, the researchers from Caen University Hospital (CHU Caen) took advantage of the PhotonIMAGER RT’s intensified CCD, which can : " dramatically increase the signal to noise ratio of NIRF signals" thanks to its fluorescence photocounting mode. NIRF acquisition times could be reduced to 22 ms, allowing
real-time in vivo NIRF imaging of freely moving mice (45 frames per second).
Using this functionality to investigate CSF circulation amid the glymphatic system hypothesis, the authors could observe a substancial increase in Indocyaning Green diffusion to the forebrain of non-anesthetized mice whilst the marker remained located in the cisterna magna of anesthetised animals. This findings, confirmed by MRI and ex vivo fluorometry analysis, strongly suggest that parenchymal CSF circulation is mainly active during wakefulness and significantly impaired during general anesthesia.
Clement Gakuba, Thomas Gaberel, Suzanne Goursaud, Jennifer Bourges, Camille Di Palma, Aurélien Quenault, Sara Martinez de Lizarrondo, Denis Vivien, Maxime Gauberti.