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Publication Alert : CMIRO, Brussels /CMMI, Gosselies, Belgium - STEM CELLS AND DEVELOPMENT

Long-term In Vivo Monitoring of Adult-Derived Human Liver Stem/Progenitor Cells by Bioluminescence Imaging, Positron Emission Tomography, and Contrast-Enhanced Computed Tomography



In this article, a triple fusion reporter system (renilla luciferase/RFP/truncated HSV-1 thymidine kinase) was used monitor biodistribution of Adult-derived human liver stem/progenitor cells (ADHLSCs) injected in severe combined immunodeficiency/beige mice by BLI FLI and Positron emission tomography.



Mei-Ju Hsu, Julie Prigent, Pierre-Edouard Dollet, Joachim Ravau, Lionel Larbanoix, Gaetan Van Simaeys, Anne Bol, Vincent Gregoire, Serge Goldman, Gisele Deblandre, Mustapha Najimi, Etienne M. Sokal, and Catherine A. Lombard.

DOI: 10.1089/scd.2016.0338

 







Publication Alert : CEA - Cyceron - Caen - PLOSOne

[18F]Fludarabine-PET in a murine model of multiple myeloma



RPMI8226-GFP-Luc MM cells expressing the green fluorescent protein (GFP) as well as the luciferase reporter (Luc) were derived from the parental RPMI8226 cells and njected s.c. into the flank of nude mice. Myeloma tumour growth was followed using bioluminescence-based imaging (BLI) and characterised by immunohistochemistry (IHC). The tumour specificity of [18F]fludarabine was evaluated and compared to [18F]FDG. The team’s results suggest that [18F]fludarabine-PET might represent an alternative and perhaps more specific modality for Multiple Myeloma imaging when compared to [18F]FDG. Nevertheless, more investigations are required to extend this conclusion to humans.


Narinee Hovhannisyan, Martine Dhilly, Martin Fidalgo, Fabien Fillesoye, Stephane Guillouet, Brigitte Sola, Louisa Barre

DOI: 10.1371/journal.pone.0177125

 







Publication Alert : Keele University - Acta Biomaterialia

Defining a turnover index for the correlation of biomaterial degradation and cell basedextracellular matrix synthesis using fluorescent tagging techniques



Non-destructive protocols which can define a biomaterial’s degradation and its associated ability to support proliferation and/or promote extracellular matrix deposition will be an essential in vitro tool. In this study we investigate fluorescently tagged biomaterials, with varying rates of degradation and their ability to support cell proliferation and osteogenic differentiation. Changes in fluorescence of the biomaterials and the release of fluorescent soluble by-products were confirmed as accurate methods to quantify degradation. It was demonstrated that increasing rates of the selected biomaterials’ degradation led to a decrease in cell proliferation and concurrently an increase in osteogenic matrix production. A novel turnover index (TI), which directly describes the effect of degradation of a biomaterial on cell behavior, was calculated. Lower TIs for proliferation and high TIs for osteogenic marker production were observed on faster degrading biomaterials, indicating that these biomaterials supported an upregulation of osteogenic markers. This TI was further validated using an ex vivo chick femur model, where the faster degrading biomaterial, fibrin, led to an increased TI for mineralization within an epiphyseal defect. This in vitro tool, TI, for monitoring the effect ofbiomaterial degradation on extracellular matrix production may well act as predictor of the selected biomaterials’ performance during in vivo studies.




Katie Bardsley, Ian Wimpenny, Roni Wechsel, Yonatan Shachaf, Ying Yang, Alicia J.El Haj

http://dx.doi.org/10.1016/j.actbio.2016.09.002






New poster of Sanofi Pasteur for AFSTAL congress in Nantes


The PhotonIMAGER system to follow bacterial infections in mice in a non-invasive manner

This Sanofi Pasteur (Marcy-l’Étoile, France) study describes two new mouse models to follow the kinetics of infection with Pseudomonas aeruginosa and Staphylococcus aureus in longitudinal studies. Thanks to the Photon IMAGERTM system daily evaluation of the bacterial load was possible in vivo. Using a luciferase reporter gene expressed in both bacterial strains, direct correlation between CFUs and light emission could be assessed non-invasively and without any need for euthanasia or organ excision.

Focusing on the 3Rs principles, Sanofi Pasteur will be aiming to apply these models to current and future infectiology studies.


 




 

Dowload the poster

Implementation of murine infectious models using in vivo optical imaging

Kevin THIBAULT-DUPREY , Thierry DECELLE, Christophe CHARNAY, Lucie ENGELHART, Vladimir PERKOV
Sanofi Pasteur, Protection et Sciences de l’Animal, Marcy-l’Étoile, France

 

 

 

 

 

New publication from Fac de Pharma in Nature Materials

The PhotonIMAGER system for in vivo Imaging of  near-infrared persistent luminescence nanoparticles

A new study of our collaborators introduce a new generation of in vivo activated optical nanoprobes based on chromium-doped zinc gallete (ZGO). In this study Biospace Lab’s PhotonIMAGER system was used for real-timme in vivo Imaging of biodistribution of ZGO nanoparticles and persistent luminescence imainging of a tumour-bearing mouse
 

 

 

       In vivo comparision of widely known QDs and ZGO nanoparticles  

Bioluminescent and fluorescent images are from 5-week old female BALB/c mice after intramuscular and systemic injection of  50 μg of either 705nm  emitting carboxyl-QDs  or ZGO nanoparticles. Shown is highly sensitive     detection of the ZGO particles in deep tissues and complete lack of autofluorescence as compared to QDs (target to background ration 186 for ZGO and 16,7 for QDs)


 

 

Take a look on the whole study of our collaborators

The in vivo activation of persistent nanophosphors for optical imaging of vascularization, tumours and grafted cells

Thomas Maldiney1, Aurélie Bessière2, Johanne Seguin1, Eliott Teston1, Suchinder K. Sharma2, Bruno Viana2, Adrie J. J. Bos3, Pieter Dorenbos3, Michel Bessodes1, Didier Gourier2, Daniel Scherman1 and Cyrille Richard1


 

 

 

 

 

A new step in Biospace Lab’s history

Biospace is pleased to announce that it is now part of the Ateliers Laumonier group

The Ateliers LAUMONIER group is a French company founded in 1923 and specializes in the development of precision tools & equipment for the Hi Tech Industry (Aerospace, nuclear power, medical equipment…)

 

This is an exciting new step for Biospace Lab, which will not only greatly benefit from over 90 years of experience in engineering and manufacturing, but also from Ateliers Laumonier’s activities in the medical and biomedical industry via their LIMMED branch.

 

Biospace Lab is also delighted to announce the launch of its new TomoFluo™ module, for the PhotonIMAGER OPTIMA system, which will be unveiled on September 17th at the WMIC 2014 in Seoul, Korea. The TomoFLuo™ module is the first module developed in partnership with The LAUMONIER group, and is designed to achieve unrivalled sensitivity & accuracy in 3D Fluorescence imaging in vivo.

New publication from UCL-CABI

The PhotonIMAGER system to image bioluminescent SHOC2 knockdown tumors in mice
A new study of our collaborators discuss an attractive target of therapeutic intervention for pharmacological inhibition of ERK pathway - the MRAS-SHOC2-PP1 holoenzyme. As a part of the study Biospace Lab’s PhotonIMAGER system were used to image bioluminescent SHOC2 knockdown tumors in mice (MDA-MB-231-EcoR-Luciferase cells infected with shRNA lentiviruses)


SHOC2 knockdown inhibits tumor formation. Bioluminescent images are from the twelfth day of mice injected with the indicated MDA-MB-231 cells. Shown is the relative bioluminescent signal of the SHOC2 knockdown tumors compared to the control tumors (shSCRAM) in the same animal. Data represent the mean± SEM of at least four tumors (∗p< 0.005).


Take a look at the whole study of our collaborators:
AnMRAS, SHOC2, and SCRIB Complex Coordinates ERK Pathway Activation with Polarity and Tumorigenic Growth
UCL Cancer Institute, University College London, London WC1E 6BT, UK.



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