Publication Alert : Keele University - Acta Biomaterialia

Defining a turnover index for the correlation of biomaterial degradation and cell basedextracellular matrix synthesis using fluorescent tagging techniques



Non-destructive protocols which can define a biomaterial’s degradation and its associated ability to support proliferation and/or promote extracellular matrix deposition will be an essential in vitro tool. In this study we investigate fluorescently tagged biomaterials, with varying rates of degradation and their ability to support cell proliferation and osteogenic differentiation. Changes in fluorescence of the biomaterials and the release of fluorescent soluble by-products were confirmed as accurate methods to quantify degradation. It was demonstrated that increasing rates of the selected biomaterials’ degradation led to a decrease in cell proliferation and concurrently an increase in osteogenic matrix production. A novel turnover index (TI), which directly describes the effect of degradation of a biomaterial on cell behavior, was calculated. Lower TIs for proliferation and high TIs for osteogenic marker production were observed on faster degrading biomaterials, indicating that these biomaterials supported an upregulation of osteogenic markers. This TI was further validated using an ex vivo chick femur model, where the faster degrading biomaterial, fibrin, led to an increased TI for mineralization within an epiphyseal defect. This in vitro tool, TI, for monitoring the effect ofbiomaterial degradation on extracellular matrix production may well act as predictor of the selected biomaterials’ performance during in vivo studies.




Katie Bardsley, Ian Wimpenny, Roni Wechsel, Yonatan Shachaf, Ying Yang, Alicia J.El Haj

http://dx.doi.org/10.1016/j.actbio.2016.09.002






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