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Biospace Lab’s digital autoradiography systems presents significant interests to complement in vivo acquisitions with

Indeed, autoradiography acquisitions only require the preparation of tissue
sections since the imagers will detect
the exact same labelling which have been used for the SPECT or PET acquisition

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Preclinical in vivo imaging of beta and positron emitting radioisotopes

Cerenkov luminescence is emitted when a charged particle passes through a living tissue at a speed greater than the phase velocity of light for the tissue. The charged particle imparts energy to molecules in the transmission medium en route and when the particle has passed by, these return rapidly to their ground state; emitting photons in the UV & continuous visible spectrum. The charged particle experiences rapid deceleration, so luminescence is confined to a region immediately around the charged particles origin. Although there are known issues with the use of short wavelength luminescence in vivo, the uses of real-time Cerenkov luminescent imaging in pre-clinical research are potentially far reaching. However, the use of Cerenkov Resonance Energy Transfer (CRET) to shift output to the near infra red has already been demonstrated‽ and this offers further scope for developing practical in vivo applications which may provide either better absolute sensitivity, or temporal resolution. Both PET and SPECT tracers are obvious Cerenkov imaging agents; however, virtually any beta emitting radioisotope with sufficient energy will generate Cerenkov luminescence in vivo , including those for which no current in vivo imaging solutions exist; such as 32P, 90Y and 90Sr.

Cerenkov luminescence in vivo

2.5 μCi of 90Y radiotracer concentrated in the pancreas of a mouse—imaged with PhotonIMAGER

Cerenkov Luminescence on the PhotonIMAGER™ systems

The intensified CCD camera technology used in both the PhotonIMAGER™ RT and the PhotonIMAGER™ Optima systems is ideally suited to imaging all types of luminescence in vivo at the highest possible sensitivity. Intensified CCD cameras acquire list mode data in real-time and without the need for the long exposure times typically required by other CCD cameras. This makes both PhotonIMAGER systems ideal for kinetic biodistribution studies using all forms of luminescence, including Cerenkov luminescence . The PhotonIMAGER system might also provide pseudo PET/SPECT real-time imaging of Cerenkov luminescence.

Although optical imaging of PET radioi-sotopes may never achieve the spatial resolution, or levels of sensitivity pro-vided by a dedicated μPET imaging system; it would make possible higher throughput experiments (i.e. 5 mice at a time) at a lower cost and, if the injected dose is sufficient, using the high temporal resolution of an iCCD camera, it should also provide the dynamic data required by applications such as real-time input function measurements.

Two frames from a real-time acquisition of Cerenkov Luminescence in vivo on the PhotonIMAGER

Left hand image is of integrated signal from the first 500 seconds following IV injection of 50 μCi 90Y. Right hand image represents the 2000 -2500 second period post injection. 90Y can be seen being excreted and collecting in the urinary bladder. This proof of principle experiment shows the potential for using Cerenkov luminescence to follow radiotracer biodistribution in vivo


Cerenkov luminescence with the 4-View module of the PhotonIMAGER™

Images of a mouse from 4 different angles 4h after the injection of 50 μCi 32P. Cerenkov luminescence signal appears in subcutaneous tumor — with a high pass (530nm) filter.

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