Photon Imager - MacroLens
The MacroLens module has been developped by Biospace Lab to open a new door to the world of bioluminescence microscopy and to build the missing link between macroscopic and microscopic acquisitions for fluorescence and bioluminescence acquisitions.
Correlate whole-body and microscopic quantificationsThe MacroLens module will give you the possibility to acquire and quantify the expression of a signal at different scales with the same instrument. This is the guaranty of a better accuracy and improved repeatability. What's more, wether you need to sacrifice the animal or not, the MacroLens module provides you with a reliable quantification at a mesoscale level before, if needed, the long and painful step of tissue section preparation for microscopy.
MicroscopyThe observation of high resolution signals in living animals was so far limited to inventive researchers who developped bench systems with cooled CCDs. Biospace Lab introduces the first
stand alone system for in vivo microscopy with the Photon Imager's MacroLens. It provides researchers with the following advantages:
- Unrivalled sensitivity. The performance of the intensified camera gives the PI a better sensitivity than cooled CCD with no trade-off on the time resolution
- Easy installation and calibration. The Photon Imager with the MacroLens module is installed and calibrated on site by Biospace Lab skilled staff
- Stand alone system. You take advantage of all the performances of the Photon Imager, including the analysis software M3 Vision for easy and precise quantification of your real time acquisitions
Many applications, like metastases studies, cell migrations or stem cell implantations, require information at different levels of detail. The MacroLens module aims
at providing a solution to this so-far unanswered challenge:
- Track your cells in the entire animal noninvasively: the macroscopic level
- Assess growth or migration spreading on a given spot at the level of the organ
- Focus on groups of cells with the microscopic level
Calcium imaging in drosophila
A transgenic drosophila was imaged with a high resolution add-on during a pulse of nicotine which induces a calcium wave in the fly brain. (Objective: 20x; field of view:0.9 mm) (A) Different areas of the brain are activated sequentially. (B) Time profile analysis of the different areas during the calcium wave. (C) Movie of nicotine induced calcium response in the drosophila mushroom bodies
Courtesy of Dr. J.R. Martin, Cellular and molecular neurobiology departement, Alfred Fressard Neurobiology Institute, CNRS, Gif sur Yvette, France.
MacroLens fluorescence acquisition of a mosquito expressing DsRed in its salivary glands, infected with GFP expressing malaria.
The malaria sporozoites first home in the insect thorax before migrating to the salivary glands of the mosquito. Such a migration is thought to be facilitated by specific chemo-attractants.
The acquisition clearly shows the GFP expression of the parasite in the insect's thorax and the DsRed expressing salivary glands. This double labeled image was overlayed with a white light picture of the mosquito, and the movie shows the two pictures overlayed with different transparency levels. Click on the image to get a larger view of the signal image.
Courtesy of Dr. Shigeto Yoshida, Department of infection and immunity, Jichi Medical school, Saimata, Japan.
Photon Imager - Optional modules
