Micro Imager



The Micro Imager:

Digital detection - High resolution
Fast acquisition - Multiple probe discrimination

Finally a digital solution to micro autoradiography

Micro Imager is 50 times faster than fil with tritium labeled samples, allows precise quantitation, and boasts a 15 µm resolution unsurpassed by any other non film technique.

Description of the detection principle of the Micro Imager

Micro Imager is fast, precise, and easy to operate

  • no complex techniques are required
  • 15 µm spatial resolution opens new fields for digital micro autoradiography
  • real-time display and list mode data file storage avoid risk of over and under exposure
  • image registration is available using either direct white light sample illumination or image import from microscope
  • an exclusive patent allows simultaneous imaging and discrimination of multiple emitters
  • precise quantitation can be performed even at very short acquisition times

Accurate analysis of labeled molecules in tissue sections and other applications

  • ex vivo positron and gamma emitter imaging
  • in situ hybridization
  • receptor-ligand
  • binding assays
  • metabolic tracer experiments
  • BNA arrays
  • biochips

The very high resolution of the Micro Imager opens a wide range of applications in

> Genomics




In situ hybridization study in rat hyppocampus using a 3H labeled probe.




Micro array involving 220 clones at 300 μm pitch on a 5 mm x 5 mm chip. The 33P probe was prepared from rat brain cells, labeled and hybridized to the corresponding cDNAs on the slide.
Courtesy of S. Dumas and J. Mallet, Laboratory of Neurotransmission Molecular Genetics and Neurodegenerative Processes (LGN), la Pitié Salpêtrière Hospital, Paris, France.

> Tissue sections




Receptor binding assay: characterization of serotonin receptors on a rat brain section, with 125I labeled serotonin-O-carboxymethyl-glycile. The image show an heterogeneous distribution with regions rich in 5-HT 1B and 5HT 1D binding sites. The absence of saturation enables distinguishing all structures in the section.
Courtesy of L. Ségu, CNRS URA 339, Bordeaux, France.







Tritium labeled rat kidney section
Courtesy of S. Harris, Roche, Welwyn, United Kingdom.












Choroid membrane of a rat eye (14C label).
Couresty of Dr. Delbos, Laboratoires Serviers, Orléans, France