Gamma Imager
Application notes
In vivo SPECT imaging and quantitation of
tumor angiogenesis in mice with the ανβ3
specific tracer 99mTc-RAFT-RGD
Lucie Sancey, Laurent Riou, Mitra Ahmadi, Daniel Fagret, Jean-Philippe Vuillez (INSERM E0340, Grenoble), Sébastien Teysseyre (Biospace Lab, Paris)
Tumor growth and metastasis are highly dependent upon neoangiogenesis. The noninvasive imaging of neovessels could therefore provide invaluable information about tumor development and treatment efficiency. Neovessels express several specific endothelial markers such as the ανβ3 integrin. RAFT-RGD is a new tracer targeted at the ανβ3 integrin and consisting of 4 cyclo(RGDfK) peptides bound to a cyclodecapeptide. In the present study, we sought to evaluate the potential of 99mTc-labeled RAFT-RGD (99mTc-RAFT-RGD) for...
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Lucie Sancey, Laurent Riou, Mitra Ahmadi, Daniel Fagret, Jean-Philippe Vuillez (INSERM E0340, Grenoble), Sébastien Teysseyre (Biospace Lab, Paris)
Tumor growth and metastasis are highly dependent upon neoangiogenesis. The noninvasive imaging of neovessels could therefore provide invaluable information about tumor development and treatment efficiency. Neovessels express several specific endothelial markers such as the ανβ3 integrin. RAFT-RGD is a new tracer targeted at the ανβ3 integrin and consisting of 4 cyclo(RGDfK) peptides bound to a cyclodecapeptide. In the present study, we sought to evaluate the potential of 99mTc-labeled RAFT-RGD (99mTc-RAFT-RGD) for...
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In vivo quantitation of a 125I-benzamide
derivative uptake within B16 melanoma
subcutaneously implanted in mice.
Correlation with quantitative autoradiographic
method
Elisabeth Miot-Noirault, Janine Papon, Jean-Michel Chezal, Jean-Claude Madelmont, Nicole Moins (UMR484 INSERM / UdA / CJP), Sébastien Teysseyre (Biospace Lab, Paris)
In the course of our investigations aimed at improving the biological characteristics of compounds having a melanoma specific affinity (benzamide series), many derivatives have been designed and assessed on the basis of their biodistribution properties in B16 melanoma bearing mice. The objective of the present study was to quantify in vivo the melanoma uptake (expressed in percentage of injected dose/g of tumor) of a new derivative (called U4841) radiolabelled with 125I. We therefore compared tumoral uptake values with...
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Elisabeth Miot-Noirault, Janine Papon, Jean-Michel Chezal, Jean-Claude Madelmont, Nicole Moins (UMR484 INSERM / UdA / CJP), Sébastien Teysseyre (Biospace Lab, Paris)
In the course of our investigations aimed at improving the biological characteristics of compounds having a melanoma specific affinity (benzamide series), many derivatives have been designed and assessed on the basis of their biodistribution properties in B16 melanoma bearing mice. The objective of the present study was to quantify in vivo the melanoma uptake (expressed in percentage of injected dose/g of tumor) of a new derivative (called U4841) radiolabelled with 125I. We therefore compared tumoral uptake values with...
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Dynamic SPECT imaging of tumor xenografts in nude mice
Bertrand Tavitian, Raphaël Boisgard, Matthieu Vanni (CEA-DSV INSERM 0103, Orsay), Sébastien Teysseyre (Biospace Lab, Paris)
Fast, high-resolution quantitative SPECT imaging is a powerful tool for the study of physiological processes. Optimal SPECT performance results from speed/resolution compromises and from the implementation of appropriate reconstruction algorithms suited to the low statistics of small animal imaging. The Gamma Imager-S (Biospace Lab, Paris) has been designed for SPECT imaging of rat and mouse models. It relies on the Gamma Imager (Biospace Lab, Paris), a scintigraphy camera with a unique continuous crystal and position sensitive photomultiplier detection head, leading to an intrinsic resolution of 2 mm and a 10 cm field of view. The Gamma Imager-S is a SPECT upgrade of the Gamma Imager, tested here in a single head version (a double head version is available). The Gamma Imager is mounted on a 2 position stand, set in the horizontal position and equipped with a rotating platform whose axis can be set at a distance from the parallel hole collimator ranging from 2 cm in the present case (mouse imaging) to 5 cm (rat imaging). The animal bed rotates at a speed of 1 turn/minute allowing dynamic tomographic reconstruction which is calculated by an OSEM (Ordered Subset Expectation Maximisation) algorithm and displayed in real time. We have tested the Gamma Imager-S in various configurations to assess...
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Bertrand Tavitian, Raphaël Boisgard, Matthieu Vanni (CEA-DSV INSERM 0103, Orsay), Sébastien Teysseyre (Biospace Lab, Paris)
Fast, high-resolution quantitative SPECT imaging is a powerful tool for the study of physiological processes. Optimal SPECT performance results from speed/resolution compromises and from the implementation of appropriate reconstruction algorithms suited to the low statistics of small animal imaging. The Gamma Imager-S (Biospace Lab, Paris) has been designed for SPECT imaging of rat and mouse models. It relies on the Gamma Imager (Biospace Lab, Paris), a scintigraphy camera with a unique continuous crystal and position sensitive photomultiplier detection head, leading to an intrinsic resolution of 2 mm and a 10 cm field of view. The Gamma Imager-S is a SPECT upgrade of the Gamma Imager, tested here in a single head version (a double head version is available). The Gamma Imager is mounted on a 2 position stand, set in the horizontal position and equipped with a rotating platform whose axis can be set at a distance from the parallel hole collimator ranging from 2 cm in the present case (mouse imaging) to 5 cm (rat imaging). The animal bed rotates at a speed of 1 turn/minute allowing dynamic tomographic reconstruction which is calculated by an OSEM (Ordered Subset Expectation Maximisation) algorithm and displayed in real time. We have tested the Gamma Imager-S in various configurations to assess...
Read more...
Improved apoptosis imaging in tumor bearing
mice by 99mTc-AFIM planar scintigraphy
Françoise Russo-Marie, Alain Samson (Bionexis, Saclay), Sébastien Teysseyre (Biospace Lab, Paris)
The objective of the present study is to assess the biodistribution of a new 99mTc-labeled radiotracer in order to detect and quantify apoptosis in tumors following chemotherapy. An early event in apoptosis is redistribution of phosphatidylserine (PS) from the inner or outer leaflet of the cell membrane. We have designed a 9 kDa miniprotein, AFIM, engineered from the annexins' domains topology and knowledge of specific PS binding sites. AFIM has a high thermodynamic stability, reversible folding, and a strong affinity for PS-containing membranes. We have engineered a multipurpose site both on AFIM and Annexin 5 that can be used either for...
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Françoise Russo-Marie, Alain Samson (Bionexis, Saclay), Sébastien Teysseyre (Biospace Lab, Paris)
The objective of the present study is to assess the biodistribution of a new 99mTc-labeled radiotracer in order to detect and quantify apoptosis in tumors following chemotherapy. An early event in apoptosis is redistribution of phosphatidylserine (PS) from the inner or outer leaflet of the cell membrane. We have designed a 9 kDa miniprotein, AFIM, engineered from the annexins' domains topology and knowledge of specific PS binding sites. AFIM has a high thermodynamic stability, reversible folding, and a strong affinity for PS-containing membranes. We have engineered a multipurpose site both on AFIM and Annexin 5 that can be used either for...
Read more...
