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Molecular Imaging for Studies of the Central Nervous System |
The following examples illustrate the use of the Photon Imager for studies of the Central Nervous System in small animals.
Focus on: Cell migration follow up in the brain of transgenic mice
The example below illustrates the possibility to track migrating cells in the brain of the living mouse. In this case, the mouse has fluorescent neuronal precursors, which migrate from the subventricular zone to the olfactory bulb through the rostral migration stream (RMS). The cells are detected in the subventricular zone and in the olfactory bulb. Such acquisitions could be performed without craniotomy, id est the signal was detected through the intact skull of the animal.
Whole body imaging of mouse with fluorescent stem cells and control mouse.
The migration pathway of neuronal precursors
Focus on the mouse brain: the cells are detected in the subventricular zone and in the olfactory bulb.
Brain imaging application examples in mice and rats
Brain Imaging - Stem cell migration
C17.2 stem cells were inoculated in the left hemisphere of the brain of this mouse. C17.2 cells, a multipotent cell line, were derived from neonatal mouse cerebellum. Such cells have been shown capable of integration in adult mouse neocortices that suffered from apoptotic degeneration.
Courtesy of I. Que, E. Kajizel, C. Löwik, LUMC, Leiden, Netherlands
C17.2 stem cells were inoculated in the left hemisphere of the brain of this mouse. C17.2 cells, a multipotent cell line, were derived from neonatal mouse cerebellum. Such cells have been shown capable of integration in adult mouse neocortices that suffered from apoptotic degeneration.
Courtesy of I. Que, E. Kajizel, C. Löwik, LUMC, Leiden, Netherlands
Imaging of neuronal precursors in utero
This mouse model has luciferase expressing neuron precursors. The bioluminescent signal could be detected in the developing brains of mouse embryos during the mouse pregnancy.
Courtesy of R. Boisgard, CEA/SHFJ.
This mouse model has luciferase expressing neuron precursors. The bioluminescent signal could be detected in the developing brains of mouse embryos during the mouse pregnancy.
Courtesy of R. Boisgard, CEA/SHFJ.
Fluorescence imaging of neuronal precursors
Neuron precursors migrate from the subventricular zone to the olfactory bulb in the mouse brain (left mouse). The acquisition was performed without craniotomy, the cells could be detected through the skull of the animal, after shaving.
The right mouse was used as control.
Courtesy of the University of Regensburg.
Neuron precursors migrate from the subventricular zone to the olfactory bulb in the mouse brain (left mouse). The acquisition was performed without craniotomy, the cells could be detected through the skull of the animal, after shaving.
The right mouse was used as control.
Courtesy of the University of Regensburg.
Fluorescence imaging of neuronal precursors
The acquisition focused on the signal of the mouse brain; the signal was co-registered with a mouse brain photography for co-localization. The detected signal is consistent with the presence of large number of neuron precursors in the subventricular zone and in the olfactory bulb.
Courtesy of the University of Regensburg.
The acquisition focused on the signal of the mouse brain; the signal was co-registered with a mouse brain photography for co-localization. The detected signal is consistent with the presence of large number of neuron precursors in the subventricular zone and in the olfactory bulb.
Courtesy of the University of Regensburg.
Monitoring of brain cancer therapy
Researchers of the Jikei University School of Medicine developped an ultra-sound base therapy which aims at killing tumor cells. This acquisition was performed to monitor and quantify the effect of the therapy on the tumor cells injected in the rat brains.
Courtesy of Dr. Yoshinobu Manome, Jikei University School of Medicine, Tokyo, Japan
Researchers of the Jikei University School of Medicine developped an ultra-sound base therapy which aims at killing tumor cells. This acquisition was performed to monitor and quantify the effect of the therapy on the tumor cells injected in the rat brains.
Courtesy of Dr. Yoshinobu Manome, Jikei University School of Medicine, Tokyo, Japan
