Betamicroprobe
Application note
Blood input function measurements with the Beta
Microprobe
Laurent Besret (URA CEA-CNRS 2210/SHFJ, Orsay), Frédéric Pain (CNRS/IPN/IPNO, Orsay)
Many important studies in live animals require intravenous injection of a radioactive tracer. To extract relevant biochemical information, time vs. tracer activity curves in physiological regions of interest are developed on the basis of compartmental models that describe the biochemical pathways of the tracer from blood to biological target. These models involve time vs. activity curves in plasma - also called “input function”- that describe the availability of the tracer to the regions of interest.
Usually the input function is measured by repeated blood sampling. In small animals this involves labor-intensive manual withdrawals that present several drawbacks. Time resolution is limited to 5-10 s between each sample, thereby affecting accuracy. Excessive amounts of blood are collected. Repeated exposure to radioactivity of those performing the sampling is also undesirable.
Therefore, we have evaluated real time measurement of the input function with a new system, the Beta MicroprobeTM (Biospace Lab, Paris). With one radiosensitive probe inserted in the femoral artery of a rat and a second placed at a remote site for use as a background control, we have obtained...
Read more...
Laurent Besret (URA CEA-CNRS 2210/SHFJ, Orsay), Frédéric Pain (CNRS/IPN/IPNO, Orsay)
Many important studies in live animals require intravenous injection of a radioactive tracer. To extract relevant biochemical information, time vs. tracer activity curves in physiological regions of interest are developed on the basis of compartmental models that describe the biochemical pathways of the tracer from blood to biological target. These models involve time vs. activity curves in plasma - also called “input function”- that describe the availability of the tracer to the regions of interest.
Usually the input function is measured by repeated blood sampling. In small animals this involves labor-intensive manual withdrawals that present several drawbacks. Time resolution is limited to 5-10 s between each sample, thereby affecting accuracy. Excessive amounts of blood are collected. Repeated exposure to radioactivity of those performing the sampling is also undesirable.
Therefore, we have evaluated real time measurement of the input function with a new system, the Beta MicroprobeTM (Biospace Lab, Paris). With one radiosensitive probe inserted in the femoral artery of a rat and a second placed at a remote site for use as a background control, we have obtained...
Read more...
