Beta Imager
Application note
Ex vivo control of visual stimuli in rat
brains by 18FDG imaging
Laurent Besret, Anne-Sophie Hérard (URA CEA-CNRS 2210, Orsay), Fabrice Beau (CEA-DSV, Orsay), Serge Maîtrejean (Biospace Lab, Paris)
In vivo imaging is rapidly developing as a direct consequence of improvements in dedicated instrumentation, superimposed upon decades of experience of in vitro imaging of tissues and molecular processes obtained, respectively, from histopathology and autoradiography. Examples are the strong association of in vivo optical microscopy with histopathology or PET/SPECT imaging with digital autoradiography. Ex vivo imaging of PET-labeled tissue sections after in vivo PET experiments is of particular interest for the precise measurement of molecular concentration or for tissue identification at high spatial resolution. While PET imaging now reaches voxel resolution in the (1-2 mm) range, pixel resolution in tissue sections by ex vivo imaging is one to two orders of magnitude better - in the (10-500µm) range depending on the technique used.
The present study was focused on the increase of FDG uptake in the left colliculus of the brain after lateral visual stimulus. One goal was to evaluate the resolution and contrast performance of popular techniques available for ex vivo imaging of beta+-labeled tissue sections. Tested were film, phosphor imaging, and two real-time digital imagers, the Micro ImagerTM and the Beta ImagerTM, both products of Biospace Lab, Paris. The comparisons were carried out on brain tissue sections for which accurate localization is of particular interest.
Read more...
Laurent Besret, Anne-Sophie Hérard (URA CEA-CNRS 2210, Orsay), Fabrice Beau (CEA-DSV, Orsay), Serge Maîtrejean (Biospace Lab, Paris)
In vivo imaging is rapidly developing as a direct consequence of improvements in dedicated instrumentation, superimposed upon decades of experience of in vitro imaging of tissues and molecular processes obtained, respectively, from histopathology and autoradiography. Examples are the strong association of in vivo optical microscopy with histopathology or PET/SPECT imaging with digital autoradiography. Ex vivo imaging of PET-labeled tissue sections after in vivo PET experiments is of particular interest for the precise measurement of molecular concentration or for tissue identification at high spatial resolution. While PET imaging now reaches voxel resolution in the (1-2 mm) range, pixel resolution in tissue sections by ex vivo imaging is one to two orders of magnitude better - in the (10-500µm) range depending on the technique used.
The present study was focused on the increase of FDG uptake in the left colliculus of the brain after lateral visual stimulus. One goal was to evaluate the resolution and contrast performance of popular techniques available for ex vivo imaging of beta+-labeled tissue sections. Tested were film, phosphor imaging, and two real-time digital imagers, the Micro ImagerTM and the Beta ImagerTM, both products of Biospace Lab, Paris. The comparisons were carried out on brain tissue sections for which accurate localization is of particular interest.
Read more...
