Photon Imager
Image Atlas
The images below illustrates the applications of the Photon Imager in the field of oncology: metastases detection, tumor growth, angiogenesis, tumor metabolism;
cell migration, brain imaging, embryo imaging, time-delayed fluorescence.
Renal carcinoma
Renal carcinoma cells (RC-21) were inoculated into the right side of the kidney capsules. Metastases could be observed in both kidneys and ovaries after 6 weeks
Courtesy of I. Que, E. Kajizel and C. Löwik, LUMC, Leiden, Netherlands
Renal carcinoma cells (RC-21) were inoculated into the right side of the kidney capsules. Metastases could be observed in both kidneys and ovaries after 6 weeks
Courtesy of I. Que, E. Kajizel and C. Löwik, LUMC, Leiden, Netherlands
Time-delayed fluorescence with optical nanoparticles
After UV excitation, the particles were injected in the animal and emitted light during several minutes based on the time delayed fluorescence principle. This technique gives the best of both worlds: the high contrast of bioluminescence and great signal of fluorescence.
Le Masne de Chermont et al, Nanoprobes with near-infrared persistent luminescence for in vivo imaging, Proc Natl Acad Sci USA, 2007 (May) (link)
After UV excitation, the particles were injected in the animal and emitted light during several minutes based on the time delayed fluorescence principle. This technique gives the best of both worlds: the high contrast of bioluminescence and great signal of fluorescence.
Le Masne de Chermont et al, Nanoprobes with near-infrared persistent luminescence for in vivo imaging, Proc Natl Acad Sci USA, 2007 (May) (link)
Bioluminescence tumor imaging
2 weeks after the IV injection of the tumoral PC12 cells, tumors were imaged in the lungs of the animal.
Courtesy of R. Boisgard, CEA/SHFJ
2 weeks after the IV injection of the tumoral PC12 cells, tumors were imaged in the lungs of the animal.
Courtesy of R. Boisgard, CEA/SHFJ
In vivo mouse embryo imaging
Luciferase expression in neuronal precursors in mouse embryo developing brains
Dr. S. Couillard Despres and Pr. L. Aigner, Univ. Regensburg, Germany
Luciferase expression in neuronal precursors in mouse embryo developing brains
Dr. S. Couillard Despres and Pr. L. Aigner, Univ. Regensburg, Germany
Brain Imaging - Stem cell migration
Inoculation of C17.2 LacZ-luc stem cells in the left hemisphere of the brain.
Courtesy of I. Que, E. Kajizel, C. Löwik, LUMC, Leiden, Netherlands
Inoculation of C17.2 LacZ-luc stem cells in the left hemisphere of the brain.
Courtesy of I. Que, E. Kajizel, C. Löwik, LUMC, Leiden, Netherlands
Cytomegalovirus expressing GFP
Right and left: mice infected by CMV expressing GFP.Center: control mouse.
Right and left: mice infected by CMV expressing GFP.Center: control mouse.
Imaging of cells after inoculation in the heart
PC3 Pro4 cells were injected in the left ventricule of the heart (150 000 cells). Acquisition tool place 20 minutes after injection.
Courtesy of I. Que, G. Van Der Pluijm, C. Löwik, LUMC, Leiden, Netherlands
PC3 Pro4 cells were injected in the left ventricule of the heart (150 000 cells). Acquisition tool place 20 minutes after injection.
Courtesy of I. Que, G. Van Der Pluijm, C. Löwik, LUMC, Leiden, Netherlands
Neuron genesis imaging
GFP expression in neuronal precursors of a C57 black/6 mouse (control mouse on the right).
GFP expression in neuronal precursors of a C57 black/6 mouse (control mouse on the right).
Tumor metabolism
In vivo imaging of a tumor using VisEn Medical optical probe ProSense. Fluorescence intensity reveals the tumor protease activity.
Courtesy of F. Ducongé, CEA/SHFJ, Orsay, France.
In vivo imaging of a tumor using VisEn Medical optical probe ProSense. Fluorescence intensity reveals the tumor protease activity.
Courtesy of F. Ducongé, CEA/SHFJ, Orsay, France.
Metastases detection
30 sec. acquisition of ganglion metastases.
Courtesy of F. Ducongé, CEA/SHFJ, Orsay, France.
30 sec. acquisition of ganglion metastases.
Courtesy of F. Ducongé, CEA/SHFJ, Orsay, France.
Ca2+ responses during keinate induced seizure
Ca2+ responses in a 7 day-old mtGA-expressing mouse. The mouse was injected with kainic acid intra peritoneally (25 mg/Kg). Imaging started right after injection. The calcium waves activated the Aequorin-GFP BRET mechanism, resulting in light production direclty linked with the propagation of the calcium transients. Calcium release was observed to reach a peak 15 minutes after kainate injection.
From Rogers et al: Non-invasive in vivo imaging of calcium signaling in mice, PLoS ONE, 2(10): e974 (2007) (link)
Ca2+ responses in a 7 day-old mtGA-expressing mouse. The mouse was injected with kainic acid intra peritoneally (25 mg/Kg). Imaging started right after injection. The calcium waves activated the Aequorin-GFP BRET mechanism, resulting in light production direclty linked with the propagation of the calcium transients. Calcium release was observed to reach a peak 15 minutes after kainate injection.
From Rogers et al: Non-invasive in vivo imaging of calcium signaling in mice, PLoS ONE, 2(10): e974 (2007) (link)
Use of this product to practice in vivo imaging in animals
(the dection of light emitted from within animals) does not imply or convey
a license to practice in vivo imaging in animals, as covered by patents controlled by other corporations.
Biospace Lab disclaims any and all responsibility for,
or liability with regard to, another Corporation's patent rights or licenses.