Micro Imager
Image Atlas
11C : ex vivo imaging after in vivo PET experiments
10 minute acquisition of a 11C compound on a rat brain section
Courtesy of the PET center, Uppsala, Sweden
10 minute acquisition of a 11C compound on a rat brain section
Courtesy of the PET center, Uppsala, Sweden
18F: ex vivo imaging after in vivo PET experiments
6 hour acquisition of rat brain sections using 5-HT1A PET ligand: 18F-MPPF
Courtesy of Prof. Luc Zimmer, CERMEP Lyon, France.
6 hour acquisition of rat brain sections using 5-HT1A PET ligand: 18F-MPPF
Courtesy of Prof. Luc Zimmer, CERMEP Lyon, France.
Triple labelling
Infarcted myocardium from a rat, which was injected with 18FDG, 99mTc-Sestamibi and 111In-DTPPA prior to sacrifice. Distributions from each of the 3 isotopes were discriminated from one single acquisition and are shown with a different color each
Courtesy of S. Poussier.
S Poussier et al. J Nucl Cardiol: 12:229-30 2005
Infarcted myocardium from a rat, which was injected with 18FDG, 99mTc-Sestamibi and 111In-DTPPA prior to sacrifice. Distributions from each of the 3 isotopes were discriminated from one single acquisition and are shown with a different color each
Courtesy of S. Poussier.
S Poussier et al. J Nucl Cardiol: 12:229-30 2005
Dual labelling
Simultaneous imaging of the tracer 99mTc-NOET and the viability tracer 201Tl in a rat model of chronic reperfused myocardial infarction.
Courtesy of Laurent Riou, INSERM, Grenoble and Carole Lartizien, CERMEP, Lyon.
Simultaneous imaging of the tracer 99mTc-NOET and the viability tracer 201Tl in a rat model of chronic reperfused myocardial infarction.
Courtesy of Laurent Riou, INSERM, Grenoble and Carole Lartizien, CERMEP, Lyon.
Bone sections
Mouse femur sections showing a mantle-cell lymphoma tumor labeled with a 90Y-labeled anti-CD20. Comparisons were made with histological sections to assess the antibody specificity.
Courtesy Jérémy Coulot, Marcel Ricard, Service de Physique, Institut Gustave Roussy, Villejuif, France.
Mouse femur sections showing a mantle-cell lymphoma tumor labeled with a 90Y-labeled anti-CD20. Comparisons were made with histological sections to assess the antibody specificity.
Courtesy Jérémy Coulot, Marcel Ricard, Service de Physique, Institut Gustave Roussy, Villejuif, France.
ISH
ISH of a 20 days mouse embryo using a 35S labeled probe specific to actin mRNA. This was obtained after an overnight acquisition, whereas more than a week for a comparable acquisition with film. The space between fingers is about 50 μm.
Courtesy of IPN, Orsay, France.
ISH of a 20 days mouse embryo using a 35S labeled probe specific to actin mRNA. This was obtained after an overnight acquisition, whereas more than a week for a comparable acquisition with film. The space between fingers is about 50 μm.
Courtesy of IPN, Orsay, France.
Mouse section
3H labeled mouse section (overnight acquisition).
Courtesy R. d'Argy, Active Biotech, Lund, Sweden.
3H labeled mouse section (overnight acquisition).
Courtesy R. d'Argy, Active Biotech, Lund, Sweden.
Choroid membrane of a rat eye
This 14C labeled product specifically tags the choroid membrane of a rat eye.
Courtesy Dr Delbos, Laboratoires Servier, Orléans, France.
This 14C labeled product specifically tags the choroid membrane of a rat eye.
Courtesy Dr Delbos, Laboratoires Servier, Orléans, France.
ISH
In-situ hybridization with two nucleic probes labeled with 3H and 14C.
Courtesy of the Riken Brain Research Institute, Japan.
In-situ hybridization with two nucleic probes labeled with 3H and 14C.
Courtesy of the Riken Brain Research Institute, Japan.
Biodistribution studies on plants:
Arabidopsis labelled with 3H GABA (left) and 14C sucrose (right) for distribution uptake studies
The plant was labelled by short-duration pulse-chase feeding, with radiolabelled metabolites, of the petiole of detached Arabidopsis leaves. The leaves are then rapidly frozen and then freeze-dried, to preserve the location of the label. Autoradiography reveals diverse patterns of labelling within these leaves, depending on the location and extent of cellular uptake of the compound fed. Simultaneous imaging of two isotopes (3H and 14C) using the Micro Imager and Energy Dual-Labelling Software allows comparison of the uptake pattern of two different metabolites within a single sample. If a particular metabolite is fed as a control in all samples, the variation in labelling pattern can be used to assess the impact of inconsistent uptake of the feed solution due to wounding or other factors.
The leaf opposite was labelled with 3H GABA (left) and 14C sucrose (right). Separation of each isotope contribution to the image was performed with Biospace Lab Dual Energy Software.
Click on the image of the setup opposite to visualize it.
Courtesy of Dr. Kinsman and Prof. Quick, Department of Animal and Plant Sciences, University of Sheffield, UK
Arabidopsis labelled with 3H GABA (left) and 14C sucrose (right) for distribution uptake studies
The plant was labelled by short-duration pulse-chase feeding, with radiolabelled metabolites, of the petiole of detached Arabidopsis leaves. The leaves are then rapidly frozen and then freeze-dried, to preserve the location of the label. Autoradiography reveals diverse patterns of labelling within these leaves, depending on the location and extent of cellular uptake of the compound fed. Simultaneous imaging of two isotopes (3H and 14C) using the Micro Imager and Energy Dual-Labelling Software allows comparison of the uptake pattern of two different metabolites within a single sample. If a particular metabolite is fed as a control in all samples, the variation in labelling pattern can be used to assess the impact of inconsistent uptake of the feed solution due to wounding or other factors.
The leaf opposite was labelled with 3H GABA (left) and 14C sucrose (right). Separation of each isotope contribution to the image was performed with Biospace Lab Dual Energy Software.
Click on the image of the setup opposite to visualize it.
Courtesy of Dr. Kinsman and Prof. Quick, Department of Animal and Plant Sciences, University of Sheffield, UK
Biodistribution studies on plants:
Uptake of 14C carbon dioxide in a pea seed
Acquisition was performed four hours after the feeding of the seed's substending leaf with 14C carbon dioxide
Courtesy of Dr. Kinsman and Prof. Quick, Department of Animal and Plant Sciences, University of Sheffield, UK
Uptake of 14C carbon dioxide in a pea seed
Acquisition was performed four hours after the feeding of the seed's substending leaf with 14C carbon dioxide
Courtesy of Dr. Kinsman and Prof. Quick, Department of Animal and Plant Sciences, University of Sheffield, UK
